h2a2 primary antibody Search Results


h2a2 primary antibody  (Miltenyi Biotec)
97
Miltenyi Biotec h2a2 primary antibody
H2a2 Primary Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h2a2+primary+antibody/DAPI+Staining+Solution/10__1158_slash_1535___7163__mct___26___0077-127-4-16
Average 97 stars, based on 1 article reviews
h2a2 primary antibody - by Bioz Stars, 2026-09
97/100 stars
  Buy from Supplier
anti rabbit γ h2ax antibody  (Boster Bio)
94
Boster Bio anti rabbit γ h2ax antibody
Fn14 shRNA attenuates fibroblast senescence in BLM-induced PF mice. A , Representative images of IHC staining of <t>γ-H2AX</t> in lung tissue, scale bar = 50 μm. B , the expression of P21 mRNA in the lungs was detected by Real-time PCR. C-D , the expression of P21 protein in the lungs was assayed by Western blot ( n = 6–8). E , Analysis of the single-cell sequencing dataset ( GSE288887 ) from pathological fibroblasts in mouse models. F, Immunofluorescence and confocal microscopy show the colocalization of FAP (green) and P16 (red) in lung tissue in BLM-treated mice. I, Immunofluorescence and confocal microscopy show the colocalization of the FAP (green) and P21 (red) localization in lung tissue of PF mice, scale bars = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001
Anti Rabbit γ H2ax Antibody, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h2a2+primary+antibody/Anti-Phospho-Histone+H2A+(S129)+HIST1H2AB+Rabbit+Monoclonal+Antibody/pmc13013858-134-4-8
Average 94 stars, based on 1 article reviews
anti rabbit γ h2ax antibody - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
rabbit anti phospho gsk3β  (Boster Bio)
94
Boster Bio rabbit anti phospho gsk3β
Fn14 shRNA attenuates fibroblast senescence in BLM-induced PF mice. A , Representative images of IHC staining of <t>γ-H2AX</t> in lung tissue, scale bar = 50 μm. B , the expression of P21 mRNA in the lungs was detected by Real-time PCR. C-D , the expression of P21 protein in the lungs was assayed by Western blot ( n = 6–8). E , Analysis of the single-cell sequencing dataset ( GSE288887 ) from pathological fibroblasts in mouse models. F, Immunofluorescence and confocal microscopy show the colocalization of FAP (green) and P16 (red) in lung tissue in BLM-treated mice. I, Immunofluorescence and confocal microscopy show the colocalization of the FAP (green) and P21 (red) localization in lung tissue of PF mice, scale bars = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001
Rabbit Anti Phospho Gsk3β, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/h2a2+primary+antibody/Anti-Phospho-Tyrosine+Rabbit+Monoclonal+Antibody/pmc12966736-283-47-52
Average 94 stars, based on 1 article reviews
rabbit anti phospho gsk3β - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier

Image Search Results


Fn14 shRNA attenuates fibroblast senescence in BLM-induced PF mice. A , Representative images of IHC staining of γ-H2AX in lung tissue, scale bar = 50 μm. B , the expression of P21 mRNA in the lungs was detected by Real-time PCR. C-D , the expression of P21 protein in the lungs was assayed by Western blot ( n = 6–8). E , Analysis of the single-cell sequencing dataset ( GSE288887 ) from pathological fibroblasts in mouse models. F, Immunofluorescence and confocal microscopy show the colocalization of FAP (green) and P16 (red) in lung tissue in BLM-treated mice. I, Immunofluorescence and confocal microscopy show the colocalization of the FAP (green) and P21 (red) localization in lung tissue of PF mice, scale bars = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Fn14 shRNA attenuates fibroblast senescence in BLM-induced PF mice. A , Representative images of IHC staining of γ-H2AX in lung tissue, scale bar = 50 μm. B , the expression of P21 mRNA in the lungs was detected by Real-time PCR. C-D , the expression of P21 protein in the lungs was assayed by Western blot ( n = 6–8). E , Analysis of the single-cell sequencing dataset ( GSE288887 ) from pathological fibroblasts in mouse models. F, Immunofluorescence and confocal microscopy show the colocalization of FAP (green) and P16 (red) in lung tissue in BLM-treated mice. I, Immunofluorescence and confocal microscopy show the colocalization of the FAP (green) and P21 (red) localization in lung tissue of PF mice, scale bars = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: Primary antibodies used were anti-rabbit γ-H2AX antibody (1:200, Boster), anti-rabbit FAP antibody (1:200, ABclonal), anti-rabbit P21 (1:200, ABclonal), anti-rabbit P16 antibody (1:100, Abcam), anti-mouse LAMP1 antibody (1:100, Proteintech), anti-mouse GM130 antibody (1:50, Proteintech), anti-rabbit STING antibody (1:200, Proteintech), anti-rabbit Ki67 antibody (1:100, Proteintech), and rabbit anti-Fn14 (1:50, Abcam).

Techniques: shRNA, Immunohistochemistry, Expressing, Real-time Polymerase Chain Reaction, Western Blot, Single Cell, Sequencing, Immunofluorescence, Confocal Microscopy

Activation of Fn14 facilitates fibroblast senescence. A-D , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h. Cell samples were harvested after 48 h. Western blot for P53, P21, and γ-H2AX proteins expression ( n = 3). E , the expressions of Il6 , Tgfb1 , Mcp1 , Mmp2 , Mmp9 , and Mmp12 mRNA in primary fibroblasts were detected by Real-time PCR ( n = 3). F , SA-β-gal staining was performed 48 h after rTWEAK treatment, scale bar = 50 μm. G , Comet assay in rTWEAK-treated primary fibroblasts, scale bar = 10 μm. H , Immunofluorescence for Ki67 protein expression in rTWEAK-treated primary fibroblasts, scale bar = 50 μm. I-J , Effects of Fn14 activation on the cell cycle were detected by flow cytometry. * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Activation of Fn14 facilitates fibroblast senescence. A-D , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h. Cell samples were harvested after 48 h. Western blot for P53, P21, and γ-H2AX proteins expression ( n = 3). E , the expressions of Il6 , Tgfb1 , Mcp1 , Mmp2 , Mmp9 , and Mmp12 mRNA in primary fibroblasts were detected by Real-time PCR ( n = 3). F , SA-β-gal staining was performed 48 h after rTWEAK treatment, scale bar = 50 μm. G , Comet assay in rTWEAK-treated primary fibroblasts, scale bar = 10 μm. H , Immunofluorescence for Ki67 protein expression in rTWEAK-treated primary fibroblasts, scale bar = 50 μm. I-J , Effects of Fn14 activation on the cell cycle were detected by flow cytometry. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: Primary antibodies used were anti-rabbit γ-H2AX antibody (1:200, Boster), anti-rabbit FAP antibody (1:200, ABclonal), anti-rabbit P21 (1:200, ABclonal), anti-rabbit P16 antibody (1:100, Abcam), anti-mouse LAMP1 antibody (1:100, Proteintech), anti-mouse GM130 antibody (1:50, Proteintech), anti-rabbit STING antibody (1:200, Proteintech), anti-rabbit Ki67 antibody (1:100, Proteintech), and rabbit anti-Fn14 (1:50, Abcam).

Techniques: Activation Assay, Western Blot, Expressing, Real-time Polymerase Chain Reaction, Staining, Single Cell Gel Electrophoresis, Immunofluorescence, Flow Cytometry

Partially restored mitophagy alleviates the senescence of rTWEAK-treated fibroblasts. A , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h after CCCP (10 µM) intervention for 30 min. Immunofluorescence and confocal microscopy show the colocalization of Mitotracker (green) and LAMP1 (red) in rTWEAK-treated primary fibroblasts with CCCP, scale bar = 50 μm. B , SA-β-gal staining was performed 48 h after rTWEAK treatment, scale bar = 50 μm. C , Comet assay in rTWEAK-treated primary fibroblasts with CCCP, scale bar = 10 μm. D-H , Western blot for P53, P21, P16, and γ-H2AX proteins expression ( n = 3). I , Real-time PCR for Il-6 , Tgfb1 , Mcp1 , Mmp2 , Mmp9 , and Mmp12 mRNA expression. * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Partially restored mitophagy alleviates the senescence of rTWEAK-treated fibroblasts. A , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h after CCCP (10 µM) intervention for 30 min. Immunofluorescence and confocal microscopy show the colocalization of Mitotracker (green) and LAMP1 (red) in rTWEAK-treated primary fibroblasts with CCCP, scale bar = 50 μm. B , SA-β-gal staining was performed 48 h after rTWEAK treatment, scale bar = 50 μm. C , Comet assay in rTWEAK-treated primary fibroblasts with CCCP, scale bar = 10 μm. D-H , Western blot for P53, P21, P16, and γ-H2AX proteins expression ( n = 3). I , Real-time PCR for Il-6 , Tgfb1 , Mcp1 , Mmp2 , Mmp9 , and Mmp12 mRNA expression. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: Primary antibodies used were anti-rabbit γ-H2AX antibody (1:200, Boster), anti-rabbit FAP antibody (1:200, ABclonal), anti-rabbit P21 (1:200, ABclonal), anti-rabbit P16 antibody (1:100, Abcam), anti-mouse LAMP1 antibody (1:100, Proteintech), anti-mouse GM130 antibody (1:50, Proteintech), anti-rabbit STING antibody (1:200, Proteintech), anti-rabbit Ki67 antibody (1:100, Proteintech), and rabbit anti-Fn14 (1:50, Abcam).

Techniques: Immunofluorescence, Confocal Microscopy, Staining, Single Cell Gel Electrophoresis, Western Blot, Expressing, Real-time Polymerase Chain Reaction

Inhibition of the cGAS-STING signaling suppresses the senescence of rTWEAK-treated fibroblasts. A-B , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h after RU.521 (10 µM) intervention for 30 min. Western blot was conducted to examine the expression levels of cGAS, STING, p-TBK1 Ser172 , and p-IRF3 Ser396 ( n = 3). C-D , The senescence-related protein levels of P53, P21, P16, and γ-H2AX were assessed by Western blot ( n = 3). E , Immunofluorescence of Ki67 protein expression in rTWEAK-treated primary fibroblasts with RU.521, scale bar = 50 μm. F , Comet assay in rTWEAK-treated primary fibroblasts with RU.521, scale bar = 10 μm. G , Real-time PCR for Il-6 , Tgf-β , Mcp1 , Mmp2 , Mmp9 , and Mmp12 mRNA expression ( n = 3). H , SA-β-gal staining was performed 48 h after rTWEAK treatment, scale bar = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Fibroblast growth factor-inducible 14 accelerates pulmonary fibrosis by inducing fibroblast senescence in mice

doi: 10.1007/s00018-026-06161-w

Figure Lengend Snippet: Inhibition of the cGAS-STING signaling suppresses the senescence of rTWEAK-treated fibroblasts. A-B , primary fibroblasts were treated with rTWEAK (100 ng/mL) for 48 h after RU.521 (10 µM) intervention for 30 min. Western blot was conducted to examine the expression levels of cGAS, STING, p-TBK1 Ser172 , and p-IRF3 Ser396 ( n = 3). C-D , The senescence-related protein levels of P53, P21, P16, and γ-H2AX were assessed by Western blot ( n = 3). E , Immunofluorescence of Ki67 protein expression in rTWEAK-treated primary fibroblasts with RU.521, scale bar = 50 μm. F , Comet assay in rTWEAK-treated primary fibroblasts with RU.521, scale bar = 10 μm. G , Real-time PCR for Il-6 , Tgf-β , Mcp1 , Mmp2 , Mmp9 , and Mmp12 mRNA expression ( n = 3). H , SA-β-gal staining was performed 48 h after rTWEAK treatment, scale bar = 50 μm. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: Primary antibodies used were anti-rabbit γ-H2AX antibody (1:200, Boster), anti-rabbit FAP antibody (1:200, ABclonal), anti-rabbit P21 (1:200, ABclonal), anti-rabbit P16 antibody (1:100, Abcam), anti-mouse LAMP1 antibody (1:100, Proteintech), anti-mouse GM130 antibody (1:50, Proteintech), anti-rabbit STING antibody (1:200, Proteintech), anti-rabbit Ki67 antibody (1:100, Proteintech), and rabbit anti-Fn14 (1:50, Abcam).

Techniques: Inhibition, Western Blot, Expressing, Immunofluorescence, Single Cell Gel Electrophoresis, Real-time Polymerase Chain Reaction, Staining